EpiCypher是一家為表觀遺傳學(xué)和染色質(zhì)生物學(xué)研究提供高質(zhì)量試劑和工具的專業(yè)制造商。EpiCypher生產(chǎn)的重組單核小體 （H3K4me3） 由 147 個(gè)DNA堿基對(duì)纏繞在組蛋白的八聚體核心（H2A、H2B、H3.2 和 H4 各兩個(gè)）上構(gòu)成，是染色質(zhì)的基本單位。Lowary和Widom[1]鑒定的147bp 601序列對(duì)組蛋白八聚體具有高親和力，可用于核小體組裝。H3K4me3 dNuc 在組蛋白H3.2的第4位含有三甲基賴氨酸。H3K4me3在110位處有一個(gè)Cys到Ala的取代。該DNA含有一個(gè)5’biotin-TEG基團(tuán)。EpiCypher生產(chǎn)的重組單核小體適用于多種應(yīng)用，包括用作酶分析、高通量篩選和抑制劑測(cè)試、染色質(zhì)結(jié)合研究、蛋白質(zhì)-蛋白質(zhì)相互作用分析、結(jié)構(gòu)研究和效應(yīng)蛋白結(jié)合實(shí)驗(yàn)中的底物。

[1] Lowary and Widom J. Mol. Biol. (1998). PMID: 9514715

產(chǎn)品詳情

宿主來源: Human

表達(dá)系統(tǒng): E. coli & synthetic DNA

標(biāo)簽: Biotinylated

保存溫度: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws

產(chǎn)品形式: 0.977 mg/mL mononucleosome in 51.2 µL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol (27.3 µg protein, 50 µg DNA + protein)

驗(yàn)證數(shù)據(jù)

	Figure 1: Western blot data Western Analysis of H3K4me3 dNuc. Top Panel: Unmodified (EpiCypher 16-0006;  Lane 1) and H3K4me3 (Lane 2) nucleosomes were probed with an anti-H3K4me3  antibody and analyzed via ECL readout. Only the H3K4me3 sample produced a  detectable signal. Bottom Panel: Detail from Coomassie stained gel showing unmodified  (Lane 1) and H3K4me3 (Lane 2) nucleosomes.
Figure 2: Mass spec data Synthetic H3K4me3 histone analyzed by high resolution mass spectrometry.  Expected mass = 15,267.77 Da. Determined mass = 15,266.15 Da.
	Figure 3: Protein gel data Coomassie stained SDS-PAGE gel of proteins in H3K4me3 dNuc (1 µg) demonstrates  the purity of histones in the preparation. Sizes of molecular weight markers and positions  of the core histones (H2A, H2B, H3K4me3 and H4) are indicated.
	Figure 4: DNA gel data H3K4me3 dNuc resolved via native PAGE gel and stained with ethidium bromide to  visualize DNA. Lane 1: Free DNA (EpiCypher 18-0005; 100 ng). Lane 2: Intact  H3K4me3 nucleosomes (400 ng).

訂購(gòu)詳情

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